DNA Electrophoresis Protocols for Forensic Genetics (Innbundet (stive permer))

Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of PCR amplicons obtained from DNA genomic markers with different inheritance patterns. In DNA Electrophoresis Protocols for Forensic Genetics, expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It includes protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and mtDNA hypervariable regions HV1 and HV2 . Protocols for molecular identification of non-human species and mRNA profiling for body fluid identification are also included. Written in the highly successful Methods in Molecular Biology(t) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Part I: Current Protocols on Capillary Electrophoresis of Amplified DNA Fragments 1. An Overview of DNA Typing Methods for Human Identification: Past, Present, and Future Robyn Thompson, Silvia Zoppis, and Bruce McCord 2. Capillary Electrophoresis and 5-channel LIF Detection of a 26plex Autosomal STR Assay for Human Identification Carolyn R. Hill. 3. Capillary Electrophoresis of Mini-STR Markers to Genotype Highly Degraded DNA Samples Michael Coble 4. Interpretation Guidelines of a Standard Y-chromosome STR 17-plex PCR-CE Assay for Crime Casework Lutz Roewer and Maria Geppert 5. Capillary Electrophoresis of an X-Chromosome STR Decaplex for Kinship Deficiency Cases Leonor Gusmao, Cintia Alves, Iva Gomes, and Paula Sanchez-Diz 6. A 48-plex autosomal SNP GenPlex Assay for Human Individualization and Relationship Testing Carmen Tomas, Claus Borsting, and Niels Morling. 7. Typing of 49 Autosomal SNPs by Single Base Extention and Capillary Electrophoresis for Forensic Genetic Testing Claus Borsting, Carmen Tomas, and Niels Morling 8. A 34-plex Autosomal SNP Single Base Extension Assay for Ancestry Investigations Christopher Phillips, Manuel Fondevila, and Maviki Lareu 9. SNaPshot(R) Minisequencing Analysis of Multiple Ancestry-informative Y-SNPs Using Capillary Electrophoresis Maria Geppert and Lutz Roewer 10. Capillary Electrophoresis of 38 Non-coding Biallelic Mini-Indels for Degraded Samples and as Complementary Tool in Paternity Testing Rui Pereira and Leonor Gusmao 11. Capillary Electrophoresis of an 11-plex mtDNA Coding Region SNP Single Base Extension Assay for Discrimination of the most Common Caucasian HV1/HV2 Mitotype Peter M. Vallone 12. Capillary Electrophoresis of a Multiplex Reverse Transcription-Polymerase Chain Reaction to Target Messenger RNA Markers for Body Fluid Identification Cordula Haas, Erin Hanson, and Jack Ballantyne 13. DNA Extraction from Aged Skeletal Samples for STR Typing by Capillary Electrophoresis Rene Huel, Sylvain Amory, Ana Bilic, Stojko Vidovic, Edin Jasaragic, and Thomas Parsons 14. Interpretation Guidelines for Multilocus STR Forensic Profiles from Low Template DNA Samples Zoran M. Budimlija and Theresa Caragine 15. Interpretation Guidelines of Mixed STR Multilocus Electrophoretic Profiles Juan Antonio Luque 16. Capillary Electrophoresis Analysis of a 9-plex STR Assay for Canine Genotyping Barbara van Asch and Antonio Amorim 17. Capillary Electrophoresis of DNA from Cannabis sativa for Correlation of Samples to Geographic Origin Heather Miller Coyle 18. Capillary Electrophoresis of Multigene Barcoding Chloroplast Markers for Species Identification of Botanical Trace Evidence Gianmarco Ferri, Beatrice Corradini, and Milena Alu Part II: Capillary electrophoresis and DNA Sequencing 19. Capillary Electrophoresis of Big-Dye Terminator Sequencing Reactions for Human mtDNA Control Region Haplotyping in the Identification of Human Remains Marta Montesino & Lourdes Prieto 20. Capillary Electrophoresis of Human mtDNA Control Region Sequences from Highly Degraded Samples using Short mtDNA Amplicons Odile Loreille and Jodi A. Irwin 21. INTERPRETATION GUIDELINES OF MTDNA CONTROL REGION SEQUENCE ELECTROPHEROGRAMS IN FORENSIC GENETICS Manuel Crespillo 22. Capillary Electrophoresis of mtDNA Cytochrome b Gene Sequences for Animal Species Identification Adrian Linacre 23. Sequence Analysis of the Canine Mitochondrial DNA Control Region from Shed Hair Samples in Criminal Investigations Cordula Berger, B. Berger and Walther Parson Part III: Advances in Microchip Capillary Electrophoresis 24. Integrated Sample Cleanup and Microchip Capillary Array Electrophoresis for High-Performance Forensic STR Profiling Peng Liu, Susan A. Greenspoon, Stephanie H. I. Yeung, and Richard A. Mathies 25. Microchip Capillary Electrophoresis Protocol to Evaluate Quality and Quantity of mtDNA Amplified Fragments for DNA Sequencing in Forensic Genetics Coro Fernandez and Antonio Alonso. 26. Microchip Capillary Electrophoresis of Multi Locus VNTR Analysis for Genotyping of Bacillus Anthracis and Yersinia Pestis in Microbial Forensic Cases Andrea Ciammaruconi