Isolation of Bacteriophages for Fastidious Bacteria.- Isolation of Bacteriophages of the Anaerobic Bacteria Bacteroides.- Isolation of Bacteriophages for Clinically Relevant Bacteria.- In Vitro Activity against Planktonic and Biofilm Populations Assessed by Flow Cytometry.- Observation of Bacteriophage Ultrastructure by Cryo-electron Microscopy.- Bacteriophage Taxonomy: an Evolving Discipline.- Determination of the Bacteriophage Host Range – Culture Based Approach.- Recovery and Characterization of Bacteria Resisting Infection by Lytic Bacteriophage.- Guidelines to Compose an Ideal Bacteriophage Cocktail.- Interaction of Bacteriophages with Mammalian Cells.- In Vivo Bacteriophage Biodistribution.- Interaction of Bacteriophages with the Immune System: Induction of Bacteriophage-specific Antibodies.- Bacteriophage Treatment of Infected Diabetic Foot Ulcers.- Compassionate Use of Bacteriophage Therapy for Foot Ulcer Treatment as an Effective Step for Moving Toward Clinical Trials.- Bacteriophage Production in Bioreactors.- Computational Modelling of Bacteriophage Production for Process Optimization.- Methods for Bacteriophage Preservation.- Bacteriophage Production in Compliance with Regulatory Requirements.- Guidelines for Bacteriophage Product Certification.- Nano/micro Formulations for Bacteriophage Delivery.- Synthetic Biology to Engineer Bacteriophage Genomes. 

This volume details the experimental approaches suitable for isolating and characterizing bacteriophages to formulating bacteriophage medicinal products and clinical application. Chapters guide readers through regulatory compliance and safety aspects of bacteriophage therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bacteriophage Therapy: From Lab to Clinical Practice aims to ensure successful results in the further study of this vital field.