Metastasis Research Protocols

Heftet / 2016 / Engelsk

Produktdetaljer

ISBN13
9781493948871
Publisert
2016
Utgave
2. utgave
Utgiver
Vendor
Humana Press Inc.
Aldersnivå
06, P
Språk
Product language
Engelsk
Format
Product format
Heftet
Sider
250
Vekt
5018 gr
Høyde
254 mm
Bredde
178 mm
Se alle

Metastasis Research Protocols

Heftet / 2016 / Engelsk
Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together updated versions of the seminal technique that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Volume 2 presents techniques applicable at the level of living cells and tissues, and presents methodologies applicable to cell behaviour in vitro, in animal models and in mathematical constructs. The aim is the study of the interaction between cancer cells and their host/environment. The focus throughout is on the tools that have been shown to be helpful in unravelling the processes important in cancer metastasis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.
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This new edition offers updates and important new techniques for illuminating the processes underlying research on metastasis. Presents techniques applicable to living cells and tissues, to cell behavior in vitro, to animal models and mathematical constructs.
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1. In Vitro Invasion Assay Using MatrigelTM - a Reconstituted Basement Membrane PreparationDebbie M S Hall and Susan A Brooks 2. Single Cell and Spheroid Collagen Type I Invasion AssayOlivier De Wever , An Hendrix, Astrid De Boeck, Frank Eertmans, Wendy Westbroek, Geert Braems, Marc Bracke 3. Rocking Adhesion Assay System to Study Adhesion and Transendothelial Migration of Cancer CellsDeepashree Bapu, Munira Khadim and Susan A. Brooks 4. Small Cell Lung Cancer (SCLC) Cell Adhesion on E- and P-Selectin under Physiological Flow ConditionsUlrich Richter and Udo Schumacher 5. Adhesion of Tumour Cells to Matrices and EndotheliumClara M Yates, Helen M McGettrick, Gerard B Nash, and G. Ed Rainger 6. Cell Aggregation AssaysDelphine Debruyne, Tom Boterberg, and Marc E. Bracke 7. Chick Heart Invasion AssayMarc E. Bracke, Virinder S. Parmar, Anthony L. DePass, Christian V. Stevens, Barbara W. Vanhoecke, and Marc M. Mareel 8. Computer Simulation of the Metastatic ProgressionGero Wedemann, Anja Bethge, Volker Haustein, and Udo Schumacher 9. Theoretical Considerations In Using Animal Models of Metastasis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude MiceSue A. Watson and Rajendra Kumari 10. Syngeneic Murine Metastasis Models: B16 MelanomaRaffaella Giavazzi and Alessandra Decio 11. Imageable Clinically-relevant Mouse Models of MetastasisRobert M. Hoffman 12. Imaging Metastatic Cell Trafficking at the Cellular Level in vivo with Fluorescent ProteinsRobert M. Hoffman 13. Ultrasound Techniques for the Detection of Tumours and Metastases in Small AnimalsMichael Didie and Wolfram-Hubertus Zimmermann 14. The PFP/RAG2 Double Knock Out Mouse in Metastasis Research: Small Cell Lung Cancer and Prostate CancerImke Muller and Sebastian Ullrich 15. Ultrasound Guided Intracardial Injection and In-vivo Magnetic Resonance Imaging of Single Cells in Mice as a Paradigm for Haematogenous MetastasesJohannes Salamon and Kersten Peldschus 16. Magnetic Resonance Imaging of Metastases in Xenograft Mouse Models of CancerKersten Peldschus and Harald Ittrich 17. Spontaneous and Experimental Metastasis Models: Nude MiceJanet E Price 18. Identifying the Origin and Phenotype of Cells in Tumour XenograftsRosemary Jeffery, Pooja Seedhar, and Richard Poulsom
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Produktdetaljer

ISBN13
9781493948871
Publisert
2016
Utgave
2. utgave
Utgiver
Vendor
Humana Press Inc.
Aldersnivå
06, P
Språk
Product language
Engelsk
Format
Product format
Heftet
Sider
250
Vekt
5018 gr
Høyde
254 mm
Bredde
178 mm
Se alle

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