Part 1 Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms 1. Culture Transfer Techniques  2. Techniques for Isolation of Pure Cultures 3. Cultural Characteristics of Microorganisms 4. Microscopic Examination of Stained Cell Preparations 5. Microscopic Exami­na­tion of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount Part 3 Bacterial Staining 6. Preparation of Bacterial Smears 7. Simple Staining 8. Negative Staining 9. Gram Stain 10. Acid-Fast Stain 11. Differential Staining for Visualization of Bacterial Cell Structures Part 4 Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations 12. Nutritional Requirements: Media for the Routine Cultivation of Bacteria 13. Use of Differential, Selective, and Enriched Media 14. Physical Factors: Temperature 15. Physical Factors: pH of the Extracellular Environment 16. Physical Factors: Atmospheric Oxygen Requirements 17. Techniques for the Cultivation of Anaerobic Microorganisms 18. Serial Dilution—Agar Plate Procedure to Quantitate Viable Cells 19. The Bacterial Growth Curve Part 5 Biochemical Activities of Microorganisms 20. Extracellular Enzymatic Activities of Microorganisms 21. Carbohydrate Fermentation 22. Triple Sugar—Iron Agar Test 23. IMViC Test 24. Hydrogen Sulfide Test 25. Urease Test 26. Litmus-Milk Reactions 27. Nitrate Reduction Test 28. Catalase Test 29. Oxidase Test 30. Utilization of Amino Acids 31. Genus Identification of Unknown Bacterial Cultures Part 6 The Protozoa 32. Free-Living Protozoa 33. Parasitic Protozoa Part 7 The Fungi 34. Cultivation and Morphology of Molds 35. Yeast Morphology, Cultural Characteristics, and Reproduction 36. Identification of Unknown Fungi Part 8 The Viruses 37. Cultivation and Enumeration of Bacteriophages 38. Isolation of Coliphages from Raw Sewage 39. Propagation of Isolated Bacteriophage Cultures Part 9 Physical and Chemical Agents for the Control of Microbial Growth 40. Physical Agents of Control: Moist Heat 41. Physical Agents of Control: Electromagnetic Radiations 42. Chemical Agents of Control: Chemotherapeutic Agents 43. Determination of Penicillin Activity in the Presence and Absence of Penicillinase 44. Chemical Agents of Control: Disinfectants and Antiseptics Part 10 Microbiology of Food 45. Microbiological Analysis of Food Products: Bacterial Count 46. Microbial Fermentation Part 11 Microbiology of Water 47. Standard Qualitative Analysis of Water 48. Quantitative Analysis of Water: Membrane Filter Method Part 12 Microbiology of Soil 49. Microbial Populations in Soil: Enumeration 50. Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates 51. Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique

NEW! A new experiment on the Propagation of Isolated Bacteriophage Cultures has been added to the Eleventh Edition. This experiment (39) guides students to isolate bacteriophages for genetic manipulation. The genetic manipulation of bacteriophages is an important technique in current clinical research as a possible way to treat antibiotic-resistant bacterial infections. NEW! Biosafety Levels (BSLs) have been added to the Eleventh Edition to alert students to appropriate safety techniques. The organisms within this book are mostly BSL-1 with some optional BSL-2 organisms. The Eleventh Edition also reflects the most up to date safety protocols from governing bodies such as the EPA, ASM, and AOAC, better preparing students for professional lab work. NEW! Tips for Success appear in select experiments and draw attention to common mistakes and stumbling blocks in the lab. Each tip explains why specific techniques are necessary to yield accurate results, helping guide students on crucial procedural steps and maximizing the available lab media and time. NEW! Revised experiments include options for alternate media, making the experiments affordable and accessible to all sizes of lab programs. Experiment 60 has been revised to focus on the normal microbiota of human skin and the importance of hand washing. NEW! Easy-to-adapt Lab Reports include blank spaces or options for “alternate organisms” for easy customization with organisms that are readily available. MasteringMicrobiology Lab-specific Assignments are available within each student’s subscription for the Pearson online homework and assessment program. To help students prepare for each class and get the most out of lab time, instructors may assign: Pre-lab quizzes for each of the 72 experiments in the Cappuccino/Welsh Lab Manual; these consist of 10 multiple-choice questions with personalized feedback for wrong answers. Each question includes a Bloom’s taxonomy rating. MicroLab Tutor Coaching Activities combine live-action technique videos and molecular animations, when appropriate, with assessment and answer-specific feedback to coach students in how to correctly perform each lab procedure and interpret/analyze different lab results. Lab Technique Videos give students an opportunity to see techniques performed correctly and quiz themselves on lab procedures both before and after lab time. New Lab Technique videos demonstrate The Scientific Method and How to Write a Lab Report. MicroLab Practical Activities assess students’ ability to interpret and analyze important lab tests, procedures, and results before their Lab Practical. Clinical Applications are integrated into each lab to help students understand why they’re learning each technique and make the connection between lab, lecture, and possible future careers as nurses or in other allied health fields. The comprehensive coverage of core microbiology topics includes experiments in the areas of genetics, immunology, and biotechnology. A wide range of experiments progressing from simple to complex enable instructors to tailor their laboratory classes to the topics they wish to cover. Experiments use the most common and affordable laboratory materials, designed to accommodate any lab. Over 90 photographs in full color and numerous illustrations appear directly alongside the experiments, helping students visualize techniques and expected results.

A new experiment on the Propagation of Isolated Bacteriophage Cultures has been added to the Eleventh Edition. This experiment (39) guides students to isolate bacteriophages for genetic manipulation. The genetic manipulation of bacteriophages is an important technique in current clinical research as a possible way to treat antibiotic-resistant bacterial infections. Biosafety Levels (BSLs) have been added to the Eleventh Edition to alert students to appropriate safety techniques. The organisms within this book are mostly BSL-1 with some optional BSL-2 organisms. The Eleventh Edition also reflects the most up to date safety protocols from governing bodies such as the EPA, ASM, and AOAC, better preparing students for professional lab work. Tips for Success appear in select experiments and draw attention to common mistakes and stumbling blocks in the lab. Each tip explains why specific techniques are necessary to yield accurate results, helping guide students on crucial procedural steps and maximizing the available lab media and time. Revised experiments include options for alternate media, making the experiments affordable and accessible to all sizes of lab programs. Experiment 60 has been revised to focus on the normal microbiota of human skin and the importance of hand washing. Easy-to-adapt Lab Reports include blank spaces or options for “alternate organisms” for easy customization with organisms that are readily available.