This book covers the latest developments in rolling circle
amplification (RCA) technology with applications in clinical
diagnostic tests and molecular medicine. Topics covered include new
enzymes useful in RCA, techniques involving RCA for enhanced signal
amplification, novel RCA diagnostics, sensors for expediting RCA
detection, and prospective RCA-based therapeutics. This is a valuable
book for university professors and students in the field of biomedical
engineering and biomolecular pharmacology as well as R&D managers of
biotechnology and biopharmaceutical companies. Specifically, this
book: Reviews prospective RCA-based therapeutics, including
RCA-derived DNA nanoparticles that strongly bind to cancer cells
Expands readers’ understanding of sensor systems for expediting
detection of RCA products by using probe-tagged magnetic nanobeads
Maximizes reader insights into novel RCA diagnostics, such as PNA
openers-assisted RCA for detection of single target cells and in situ
RCA diagnosis of cancer cells and malignant tissues Presents
innovative methods for quasi-exponential enhancement of RCA-generated
signals, such as nicking enzyme-assisted cascade RCA and RCA coupled
with loop-mediated amplification Advance Praise for Rolling Circle
Amplification (RCA): “This book provides a badly needed compendium
of innovative RCA methods and applications. It should help further
increase the community of scientists that have employed RCA in
research and diagnostic programs.” — Charles Cantor, Professor
Emeritus of Biomedical Engineering, Boston University Executive
Director, Retrotope Inc. (USA) “In this new book Vadim Demidov has
assembled an enticing menu of articles that illustrate the evolution
of the RCA field, including improved protein parts for building
superior DNA nanomachines, enhanced modalities of amplification and
detection, diagnostic applications, and even a sampling of potential
therapeutic applications. The reader will appreciate that while RCA
has come of age, there is no lack of exciting surprises, turns, and
twists in the continuing evolution of the technology.” — Paul
Lizardi, Professor of Pathology, Yale University School of Medicine
(retired) Investigator, University of Granada, Spain, President,
PetaOmics, Inc., San Marcos, Texas.
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Produktdetaljer
ISBN
9783319422268
Publisert
2018
Utgiver
Vendor
Springer
Språk
Product language
Engelsk
Format
Product format
Digital bok