This book contains accepted papers presented at ICGEC 2024, the 16th International Conference on Genetic and Evolutionary Computing, held from August 28-29, 2024 in Miyazaki, Japan. The conference is intended as an international forum for the researchers and professionals in all areas of genetic and evolutionary computing. And the readers may know the up-to-date techniques of the mentioned topics, including digital transformation, machine learning and data analysis, meta-heuristic optimization algorithms, computer vision, and artificial intelligence of things (AIoT), which can help them to bring new ideas or apply the designed approaches from the collected papers to their professional jobs.