In this ready reference top international authors present environmentally friendly and stereoselective methods of modern biocatalysis, covering emerging topics and showing how these novel methods and concepts can be used for practical applications.

Preface DIRECTED EVOLUTION OF LIGNINOLYTIC OXIDOREDUCTASES: FROM FUNCTIONAL EXPRESSION TO STABILIZATION AND BEYOND Introduction Directed Molecular Evolution The Ligninolytic Enzymatic Consortium Directed Evolution of Laccases Directed Evolution of Peroxidases and Peroxygenases Saccharomyces cerevisiae Biomolecular Tool Box Conclusions and Outlook NEW TRENDS IN THE IN SITU ENZYMATIC RECYCLING OF NAD(P)(H) COFACTORS Introduction Recent Advancements in the Enzymatic Methods for the Recycling of NAD(P)(H) Coenzymes and Novel Regeneration Systems Conclusions MONOOXYGENASE-CATALYZED REDOX CASCADE BIOTRANSFORMATIONS Introduction Enzymatic Oxygenation Effective Cofactor Recycling In Vitro Multistep Biocatalysis Combined In Vitro and In Vivo Multistep Biocatalysis In Vivo Multistep Biocatalysis Chemo-Enzymatic Cascade Reactions Conclusions and Outlook BIOCATALYTIC REDOX CASCADES INVOLVING W-TRANSAMINASES Introduction General Features of w-Transaminases Linear Cascade Reactions Involving w-Transaminases Concluding Remarks MULTI-ENZYME SYSTEMS AND CASCADE REACTIONS INVOLVING CYTOCHROME P450 MONOOXYGENASES Introduction Physiological Cascade Reactions Involving P450s Artificial Cascade Reactions Involving P450s Conclusions and Outlook CHEMO-ENZYMATIC CASCADE REACTIOS FOR THE SYNTHESIS OF GLYCOCONJUGATES Introduction Sequential Syntheses One-Pot Syntheses Convergent Syntheses Conclusion SYNERGIES OF CHEMISTRY AND BIOCHEMISTRY FOR THE PRODUCTION OF BETA-AMINO ACIDS Introduction Dihydropyrimidinase N-Carbomoyl-Beta-Alanine Amidohydrolase Bienzymatic System for Beta-Amino Acid Production Conclusions and Outlook RACEMIZABLE ACYL DONORS FOR ENZYMATIC DYNAMIC KINETIC RESOLUTION Introduction The Tools Applications of DKR to Acyl Compounds Conclusions STEREOSELECTIVE HYDROLASE-CATALYZED PROCESSES IN CONTINUOUS-FLOW MODE Introduction Enzyme-Catalyzed Stereoselective Reactions in Continuous-Flow Systems Outlook and Perspectives PERSPECTIVES ON MULTIENZYME PROCESS TECHNOLOGY Introduction Multienzyme System Classification Biocatalyst Options Reactor Options Process Development Process Modeling Future Concluding Remarks NITRILE CONVERTING ENZYMES INVOLVED IN NATURAL AND SYNTHETIC CASCADE REACTIONS Introduction Natural Cascades Artificial Cascades Conclusions and Future Use of These Enzymes MINING GENOMES FOR NITRILASES Strategies in Nitrilase Search Diversity of Nitrilase Sequences Structure-Function Relationships Enyzme Properties and Applications Conclusions KEY-STUDY ON THE KINETIC ASPECTS OF THE IN SITU NHASE/AMASE CASCADE SYSTEM OF M. imperiale RESTING CELLS FOR NITRILE BIOCONVERSION Introduction The Temperature Effect on the NHase-Amidase Bi-Enzymatic Cascade System Effect of Nitrile Concentration on NHase Activity and Stability Effect of Nitrile on the AMase Activity and Stability Concluding Remarks ENZYMATIC STEREOSELECTIVE SYNTHESIS OF BETA-AMINO ACIDS Introduction Preparation of Beta-Amino Acids Nitrile Hydrolysis Enzymes Conclusion NEW APPLICATIONS OF TRANSKETOLASE: CASCADE REACTIONS FOR ASSAY DEVELOPMENT Introduction Cascade Reactions for Assaying Transketolase Activity In Vitro Selection Conclusion ALDOLASES AS CATALYST FOR THE SYNTHESIS OF CARBOHYDRATES AND ANALOGS Introduction Iminocyclitol and Aminocyclitol Synthesis Carbohydrates and Other Polyhydroxylated Compounds Conclusions ENZYMATIC GENERATION OF SIALCONJUGATE DIVERSITY Introduction A Generic Strategy for the Synthesis of Sialconjugate Libraries Cascade Synthesis of Neo-Sialconjugates Conclusions METHYLTRANSFERASES IN BIOCATALYSIS Introduction SAM-Dependent Methyltransferases Conclusion and Outlook CHEMOENZYMATIC MULTISTEP ONE-POT PROCESSES Introduction: Why Chemoenzymatic Cascades and Why One-Pot Processes? Concepts of Chemoenzymatic Processes Combination of Substrate Isomerization and their Derivatization with Chemo- and Biocatalysts Resulting in Dynamic Kinetic Resolutions and Related Processes Combination of Substrate Synthesis (Without Isomerization) and Derivatization Step(s) Conclusion and Outlook Index

This ready reference presents environmentally friendly and stereoselective methods of modern biocatalysis. The experienced and renowned team of editors have gathered top international authors for this book. They cover such emerging topics as chemoenzymatic methods and multistep enzymatic reactions, while showing how these novel methods and concepts can be used for practical applications. Multidisciplinary topics, including directed evolution, dynamic kinetic resolution, and continuous-flow methodology are also discussed. From the contents: * Directed Evolution of Ligninolytic Oxidoreductases: from Functional Expression to Stabilization and Beyond * New Trends in the In Situ Enzymatic Recycling of NAD(P)(H) Cofactors * Monooxygenase-Catalyzed Redox Cascade Biotransformations * Biocatalytic Redox Cascades Involving w-Transaminases * Multi-Enzyme Systems and Cascade Reactions Involving Cytochrome P450 Monooxygenases * Chemo-Enzymatic Cascade Reactions for the Synthesis of Glycoconjugates * Synergies of Chemistry and Biochemistry for the Production of Beta-Amino Acids * Racemizable Acyl Donors for Enzymatic Dynamic Kinetic Resolution * Stereoselective Hydrolase-Catalyzed Processes in Continuous-Flow Mode * Perspectives on Multienzyme Process Technology * Nitrile Converting Enzymes Involved in Natural and Synthetic Cascade Reactions * Mining Genomes for Nitrilases * Key-Study on the Kinetic Aspects of the In-Situ NHase/AMase Cascade System of M. imperiale Resting Cells for Nitrile Bioconversion * Enzymatic Stereoselective Synthesis of Beta-Amino Acids * New Applications of Transketolase: Cascade Reactions for Assay Development * Aldolases as Catalyst for the Synthesis of Carbohydrates and Analogs * Enzymatic Generation of Sialoconjugate Diversity * Methyltransferases in Biocatalysis * Chemoenzymatic Multistep One-Pot Processes