Animal cell culture is a common laboratory technique for maintaining live cells separated from their original tissue (primary cells) or immortal cells (cell lines). The technique of cell culture was discovered in the 19th century after the discovery of the microscope.

Presently, the culture of cells is one of the most important tools of life sciences research and is being used by investigators to understand advanced understanding of cell growth and differentiation and mechanisms of normal/ abnormal functioning of various cells. In addition to that, cell culture is employed in the generation of vaccines, the production of pharmaceutical proteins, and the safety testing of drugs.
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  • 1 Layout, Safety Protocols and Guidelines in Cell Culture Facilities
  • 2 Basic Equipment of Animal Cell Culture Laboratories
  • 3 Washing, Packing, and Sterilization of Glass and Plastic Wares
  • 4 Preparation of Cell Culture Media and Reagents
  • 5 Isolation of Primary Culture of Chicken Embryo Fibroblast
  • 6 Isolation of Primary Culture of Canine Keratinocytes
  • 7 Isolation of Primary Culture of Canine Fibroblast
  • 8 Culture and Sub-culturing of Continuous Cell Lines
  • 9 Trypan Blue Dye Exclusion Cell Viability Assay
  • 10 Isolation and Culture Techniques for Lymphocytes
  • 11 Cryopreservation Methods of Primary Cultures and Cell Lines
  • 12 Evaluating Cytopathic Effects of Viruses on Mammalian Cells
  • 13 Virus Isolation in Cell Culture System
  • 14 One-Step Growth Curve of a Virus
  • 15 Production of Virus in Cell Culture
  • 16 Quantitative Analysis of Virus Titers through In-Vitro Assays
  • 17 Hemadsorption (HA) Assay for Virus Titration
  • 18 Plaque Assay for Virus Detection and Quantification
  • 19 Virus Neutralization Test
  • 20 Techniques for Establishing and Maintaining Cell Suspension Cultures
  • 21 Development of Cell lines Through Virus-Mediated Techniques
  • 22 Cell Viability and Cell Proliferation Assay
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Product details

ISBN
9789358879704
Published
2024-10-08
Publisher
New India Publishing Agency
Weight
300 gr
Height
229 mm
Width
108 mm
Age
P, 06
Language
Product language
Engelsk
Format
Product format
Innbundet
Number of pages
108

Biographical note

Ratan Kumar Choudhary College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, India

Manu M College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, India

Yashpal Singh Malik: College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, India.