Preface to the Ninth Edition xv

About the Companion Website xvii

Part I The Basic Principles of Gene Cloning and DNA Analysis 1

1 Why Gene Cloning and DNA Analysis Are Important? 3

2 Vectors for Gene Cloning: Plasmids and Bacteriophages 15

3 Purification of DNA from Living Cells 29

4 Manipulation of Purified DNA 55

5 Introduction of DNA into Living Cells 87

6 Cloning Vectors for Escherichia coli 105

7 Cloning Vectors for Eukaryotes 125

8 How to Obtain a Clone of a Specific Gene 151

9 The Polymerase Chain Reaction 175

Part II The Applications of Gene Cloning and DNA Analysis in Research 193

10 Sequencing Genes and Genomes 195

11 Studying Gene Expression and Function 221

12 Studying Genomes 245

13 Studying Transcriptomes and Proteomes 261

14 DNA Editing 279

Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology 293

15 Production of Protein from Cloned Genes 295

16 Gene Cloning and DNA Analysis in Medicine 319

17 Gene Cloning and DNA Analysis in Agriculture 347

18 Gene Cloning and DNA Analysis in Forensic Science and Archaeology 375

Glossary 393

Index 413

New edition of a world-renowned introductory text to the rapidly-growing and dynamic field of Gene Cloning and DNA Analysis

Gene Cloning and DNA Analysis is a comprehensive introductory text that explores all of the topics vital for a complete understanding of gene cloning and DNA analysis. Presented in full color, the text’s easy-to-follow layout and over 250 clear illustrations make the material simple and accessible for a diverse readership.

This newly revised and updated Ninth Edition highlights new developments in DNA sequencing technology and provides extended coverage on DNA editing including CRISPR methodology and its applications in gene therapy and plant genetic engineering.

This edition also contains updates on topics including optical mapping of DNA molecules, studying transcriptomes in situ, RNA interference methods for silencing genes in crop plants, studying kinship by DNA profiling, and using ancient DNA to study human prehistory and palaeogenomics.

Gene Cloning and DNA Analysis also discusses topics such as:

• The strategies used by researchers and industry practitioners to assemble telomere-to-telomere chromosome sequences
• The synthesis of recombinant vaccines for diseases such as COVID-19 and hepatitis B
• Interbreeding between Neanderthals and Homo sapiens
• The ethical issues raised by the use of gene cloning and DNA editing in pharming, gene therapy and plant genetic modification

The Ninth Edition of Gene Cloning and DNA Analysis continues to be an essential reference for undergraduate and graduate students within the fields of genetics and genomics, molecular biology, biochemistry, immunology, and applied biology, as well as professionals in all areas of biological science.