Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses.
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1. Introduction Enzymes-Past, present and Future
2. Distribution and diversity in microbial enzymes
3. Screening of potential microbes for enzymes of industrial significance
4. Solid state fermentations and SMF for enzyme production
5. Laboratory methods for enzyme assays
6. Structure and functions of enzyme kinetics
7. Protocols of important industrial enzymes
8. Strategies to improve enzyme activity for industrial processes
9. Scope and relevance of Industrial applications
10. Agro-industrial wastes for enzyme production
11. Significance of enzymes and their agricultural applications
12. Biotechnological applications of enzymes and future prospective
13. Conclusions
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Offers thorough instruction on the experimental procedures and applications of enzymology across biochemistry, microbiology, biotechnology, and allied fields
Provides step-by-step instruction on enzyme protocols and applications, with actionable discussions of needed resources, materials, quantification and statistical analysis, optimization and troubleshooting, safety considerations and standards
Presents easy to read, reproducible protocols for researchers and students across academia and industry
Includes color diagrams that illustrate key concepts
Les mer
Produktdetaljer
ISBN
9780323912686
Publisert
2021-11-24
Utgiver
Elsevier Science & Technology
Vekt
540 gr
Høyde
235 mm
Bredde
191 mm
Aldersnivå
P, 06
Språk
Product language
Engelsk
Format
Product format
Heftet
Antall sider
266