This Laboratory Manual has been specifically created for students to simplify their understanding of fundamental plant pathological laboratory methods that are related to the isolation of pathogens. The manuals primary goal is to provide students with a concise, easy-to-understand guide to the essential concepts and techniques required for the study of plant pathology. The manual has been designed to provide a clear understanding of the various aspects of plant pathology and to advance students knowledge of the subject. The manual includes detailed information on preserving disease samples, demonstrating Kochs postulates, studying different classes of fungicides and antibiotics, preparing fungicides, applying fungicides, conducting bioassays of fungicides, and controlling plant pathogens using biological methods. The manual is written in a formal tone and contains descriptions of key terminologies to enhance students comprehension of the subject matter. The manual is a full-color book that includes digitized images to help students identify diseases and pathogens. It is an excellent resource for beginners, students, instructors, scientists, and research workers who are interested in the field of Plant Pathology and Agricultural Microbiology.