3D printing is a promising fabrication technique capable of creating objects with precise structures and shapes based on 3D model data, allowing for versatile alterations and production of intricate shapes while also reducing the consumption of feedstock, particularly crucial in the cost-sensitive realm of medicine. In the biomedical sphere the use of 3D printing spans various applications, from artificial organs to sensors and orthopedic implants. This handbook explores the transformative role of technology in healthcare, focusing on materials, design, and innovation. It covers diverse applications like prosthetics, devices, tissue engineering, and medicine. This comprehensive resource highlights cutting-edge advancements, ethical considerations, and future trends, offering insights for researchers, professionals, and students. Explores the latest advances, technologies, and practical applications of additive manufacturing in biomedical engineering, including its impact on tissue engineering, drug delivery systems, regenerative medicine, and orthodontics Covers polymer 3D bioprinting techniques and advanced materials pivotal for successful biomedical implants in clinical settings Discusses the integration of smart materials and their applications including point-of-care solutions Encompasses various facets of medical technology, including diagnostic instruments, therapeutic devices, tissue engineering, and regenerative medicine Examines both in vivo and in vitro studies related to 3D printing, unraveling their significance and implications in various contexts Considers challenges and future perspectives With its comprehensive approach, this handbook serves as the definitive resource for researchers, practitioners, and advanced students, fostering a deeper appreciation and understanding of the transformative power of 3D printing in shaping the future of biomedical engineering.