This second edition of MEMBRANE PROTEIN PURIFICATION AND
CRYSTALLIZATION, A PRACTICAL GUIDE is written for bench scientists
working in the fields of biochemistry, biology, and proteomic
research. This guide presents isolation and crystallization techniques
in a concise form, emphasizing the critical aspects unique to membrane
proteins. It explains the principles of the methods and provides
protocols of general use, permitting researchers and students new to
this area to adapt these techniques to their particular needs. This
edition is not only an update but is comprised mainly of new
contributions. It is the first monograph compiling the essential
approaches for membrane protein crystallization, and emphasizes recent
progress in production and purification of recombinant membrane
proteins.
Key features
* Provides general guidelines and strategies for isolation and
crystallization of membrane proteins
* Gives detailed protocols that have wide application, and low
specialized equipment needs
* Emphasizes recent progress in production and purification of
recombinant membrane proteins, especially of histidine-tagged and
other affinity-epitope-tagged proteins
* Summarizes recent developments of Blue-Native PAGE, a high
resolution separation technique, which is independent of the use of
recombinant techniques, and is especially suited for proteomic
analyses of membrane protein complexes
* Gives detailed protocols for membrane protein crystallization, and
describes the production and use of antibody fragments for high
resolution crystallization
* Presents a comprehensive guide to 2D-crystallization of membrane
proteins
Les mer
Produktdetaljer
ISBN
9780080536170
Publisert
2013
Utgave
2. utgave
Utgiver
Vendor
Academic Press
Språk
Product language
Engelsk
Format
Product format
Digital bok
Antall sider
316