Produktdetaljer
Biographical note
Stanford University, California, USAChristina Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer's yeast, is regarded as one of the most brilliant minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set.
Fundamentals
The first volume provides an overview of metabolic pathway engineering with a look towards the future. It discusses cellular metabolism, including transport processes inside the cell and energy generating reactions, as well as rare metabolic conversions. This volume also explores balances and reaction models, the regulation of metabolic pathways, and genome scale and multiscale modeling tools. It also covers developing appropriate hosts for metabolic engineering including the use of Escherichia coli, yeast, Bacillus subtilis, Streptomyces, filamentous fungi, annd mammalian cells using cell culture.
Tool and Applications
The second volume delves into evolutionary tools, including those associated with gene expression for metabolic pathway engineering. It covers applications of emerging technologies, including research on genome-wide technologies, DNA and phenotypic microarrays, and proteomics tools for experimentally determining flux through pathways. It also evaluates emerging applications for producing fine chemicals, drugs, and alternative fuels.
Volume I
Introduction and Preface to Handbook, Christina D. Smolke
Progression of biological synthesis methods towards commercial relevance
The field of metabolic engineering
An overview of the metabolic pathway engineering handbook
Metabolic engineering: looking towards the future
Section I. Cellular Metabolism, Vincent Martin
Transport processes inside the cell
Energy generating reactions
Biosynthesis of important building blocks
Polymerization of building blocks to macromolecules
Rare metabolic conversions - harvesting diversity through nature
Section II. Balances and Reaction Models, Walter van Gulik
Growth nutrients and diversity
Mass balances, rates, and experiments
Data reconciliation and error detection
Black box models for growth and product formation
Metabolic models for growth and product formation
A thermodynamic description of microbial growth
Section III. Regulation of Metabolic Pathways, James Liao
Regulation of specific pathways
Regulating of secondary metabolism
Building networks as assemblies of simpler control schemes
Section IV. Modeling Tools for Metabolic Engineering, Costas Maranas
Metabolic flux analysis
Metabolic control analysis
Structure and flux analysis of metabolic networks
Constraint-based genome-scale models of cellular metabolism
Multi-scale modeling of metabolic regulation
Validation of metabolic models
Section V. Developing Appropriate Hosts for Metabolic Engineering, Jens Nielsen
Escherichia coli as a well-developed host for metabolic engineering
Metabolic engineering in yeast
Metabolic engineering in Bacillus subtilis
Metabolic engineering in Streptomyces
Metabolic engineering in filamentous fungi
Metabolic engineering in mammalian cells using cell culture
Volume II
Section VI. Evolutionary Tools in Metabolic Engineering, Claudia Schmidt-Dannert
Evolutionary engineering of industrially important microbial phenotypes
Expanding enzyme function and activity through molecular evolution
Evolving the function of DNA and RNA regulatory regions through molecular evolution
Evolving pathways and genomes for the production of natural and novel compounds
Models predicting optimized evolutionary strategies
Section VII. Gene Expression Tools for Metabolic Pathway Engineering, Christina Smolke
Low-copy number plasmids as artificial chromosomes
Chromosomal engineering strategies
Regulating gene expression through engineered RNA technologies
Tools designed to regulate translational efficiency
Regulating gene expression through post-translational modifications
Engineering bifunctional enzymes for optimized metabolite transfer between sequential conversion steps
Practical pathway engineering - demonstration in integrating tools
Section VIII. Applications of Emerging Technologies to Metabolic Engineering, Jay Keasling
Genome-wide technologies: DNA and phenotypic microarrays, proteomics
Monitoring and measuring the metabolome
Section IX. Future Prospects in Metabolic Engineering, Bernhard Palsson and Sang Yup Lee
Genome-scale models, systems biology, and metabolic engineering
Cell-free systems for metabolic engineering
Dynamic in silico cell models
Appendix I: Tools for Experimentally Determining Flux through Pathways, Ralf Takors
Measuring flux by isotope labeling
Tools for measuring intermediate and product formation
Quantitative analysis of labeling data
Appendix II: Applications, Brian Pfleger and Brian Brazeau
Cofactor regulation in biocatalytic redox reactions
Metabolic engineering for fine chemical production
Metabolic engineering for drug production
Metabolic engineering for alternative fuels