Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together the most up to date versions of the seminal techniques that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Presented by top scientists, the collection includes a wide spectrum of articles encompassing important key methods and to introduce new methods which are making an impact in the area of metastasis research. Volume 1 includes key cellular and molecular techniques relevant to the exploration of cancer cells and tissues, the focus is on the tools that have been shown to be helpful in unravelling the molecular processes important in cancer metastasis. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.
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Now in a second, revised edition, this practical companion to the methodologies of researching cancer growth includes topical introductions and stepwise, reproducible protocols for laboratory processes, as well as invaluable troubleshooting tips.
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1. Basic Immunocytochemistry for Light MicroscopySusan A. Brooks2. Lectin Histochemistry to Detect Altered Glycosylation in Cells and TissuesSusan A. Brooks & Debbie M.S. Hall3. Histopathological Assessment of MetastasisDerek E Roskell and Ian D Buley 4. Immunocytochemical Detection and Characterisation of Individual Micrometastatic Tumour CellsStephan Braun and Klaus Pantel5. The Polymerase Chain Reaction (PCR)Hazel M. Welch6. SDS-PAGE and Western Blotting TechniquesChristine Blancher and Rob Mc Cormick7. 2-DE Based Proteomics for the Analysis of Metastasis Associated Proteins Miriam V Dwek and Diluka Peiris8. Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin ZymographyMarta Toth, Anjum Sohail, and Rafael Fridman9. Determination of Cell Specific Receptor Binding using a Combination of Immunohistochemistry and in vitro Autoradiography: Relevance to Therapeutic Receptor Targeting in CancerMichael R. Dashwood and Marilena Loizidou10. Fluorescence in situ Hybridization (FISH) for Cancer Related Studies Lyndal Kearney and Janet Shipley11. Using a Quartz Crystal Microbalance Biosensor for the Study of Metastasis Markers on Intact Cells Julien Saint Guirons and Bjoern Ingemarsson12. Cell Separations by Flow CytometryDerek Davies13. Detection of Putative Cancer Stem Cells of the Side Population Phenotype in Human Tumor Cells CulturesMatthias Christgen, Matthias Ballmaier, Ulrich Lehmann and Hans Kreipe14. Genes Involved in the Metastatic Cascade of Medullary Thyroid TumoursCaroline Schreiber, Kirsten Vormbrockand Ulrike Ziebold15. High-resolution Quantitative Methylation Analysis of microRNA Genes using Pyrosequencing (TM)Ulrich Lehmann, Cord Albat, Hans Kreipe 16. RNAi Technology to Block the Expression of Molecules Relevant to Metastasis: The Cell Adhesion Molecule CEACAM1 as an Instructive ExampleDaniel Wicklein 17. Galectin-3-Binding and MetastasisPratima Nangia-Makker, Vitaly Balan, and Avraham Raz 18. Lectin Array Based Strategies for Identifying Metastasis-associated Changes in GlycosylationSimon Fry, Babak Afrough, Anthony Leathem and Miriam V Dwek
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From the book reviews:"This second edition ... describes cellular and molecular techniques used to study cancer metastasis. ... It is written for any researchers interested in cellular and molecular techniques relevant to cancer cells and tissue. ... This is a great addition to any laboratory studying cancer metastasis. ... I plan to have all my new lab members read through this book. Even those already working for years in the lab can learn new tips and techniques." (Jennifer E. Koblinski, Doody's Book Reviews, January, 2013)
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2. utgave
Humana Press Inc.
743 gr
254 mm
178 mm
06, P
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