This laboratory manual includes the latest tools and techniques involved in genomic research. It starts with an introductory chapter on genomics and the various tools and applications involved. The initial chapters present protocols for basic techniques such as DNA isolation, electrophoresis, PCR, cDNA synthesis etc. The book then goes on to describe more advanced techniques such as next-generation sequencing, exome sequencing, use of RNAi, RNAseq, genome editing, single cell genomics etc. Each topic includes a brief description, information on the principles involved, materials & methods, protocol, and expected results, with diagrams and graphs. All protocols are presented in a very lucid and precise way, to make it easy for readers to follow and replicate them.
This laboratory manual includes the latest tools and techniques involved in genomic research. The book then goes on to describe more advanced techniques such as next-generation sequencing, exome sequencing, use of RNAi, RNAseq, genome editing, single cell genomics etc.
Chapter 1: Conventional and Basic genomic techniques.- Chapter 2: Recombinant DNA technology.- Chapter 3: Mitochondrial Genetics.- Chapter 4: Basic principle of Cloning.- Chapter 5: Immunohistochemistry.- Chapter 6: Western blotting.- Chapter 7: Introduction to Bioinformatics.- Chapter 8: Molecular evolutionary Study-Phylogenetic tree.- Chapter 9: Real Time or Quantitative PCR.- Chapter 10: Tissue or Cell Cultures.- Chapter 11: DNA Micro Array.- Chapter 12: Epigenetics and DNA Methylation.- Chapter 13: RNA sequencing.- Chapter 14: Next Generation sequencing.- Chapter 15: Metagenomics.- Chapter 16: Genome wide association studies (GWAS)/ SNP chips.- Chapter 17: Exome sequencing.- Chapter 18: RNA Interference.- Chapter 19: Expressing cloned genes for protein production, purification and analysis-Biopharming.- Chapter 20: Transgenesis and Biopharming.- Chapter 21: Rapid amplification of cDNA ends (RACE).- Chapter 22: Gene Therapy.- Chapter 23: Nutrigenomics.- Chapter 24: Gene Editing.- Chapter 25: Introduction to stem cell biology.- Chapter 26: Transcriptomics.- Chapter 27: Development of Biomarker.- Chapter 28: Single cell genomics.- Chapter 29: Big data Analysis.
Springer-Verlag New York Inc.