Introduction I. MICROSCOPY 1. Use and Care of the Microscope 2. Examination of Living Microorganisms II. Handling Bacteria 3. Microbes in the Environment 4. Transfer of Bacteria: Aseptic Techniques III. STAINING METHODS 5. Preparation of Smears and Simple Staining 6. Negative Staining 7. Gram Staining 8. Acid-Fast Staining 9. Structural Stains (Endospore, Capsule, Flagella) 10. Morphologic Unknown IV. CULTIVATION OF BACTERIA   11. Isolation of Bacteria by Dilution Techniques 12. Special Media for Isolating Bacteria V. MICROBIAL METABOLISM 13. Carbohydrate Catabolism 14. Fermentation 15. Protein Catabolism, Part 1 16. Protein Catabolism, Part 2 17. Respiration 18. Unknown Identification and Bergey’s Manual VI. MICROBIAL GROWTH 19. Oxygen and the Growth of Bacteria 20. Determination of a Bacterial Growth Curve: The Role of Temperature 21. Biofilms   VII. CONTROL OF MICROBIAL GROWTH 22. Physical Methods of Control: Heat 23. Physical Methods of Control: Ultraviolet Radiation 24. Chemical Methods of Control: Disinfectants and Antiseptics 25. Chemical Methods of Control: Antimicrobial Drugs 26. Effectiveness of Hand Scrubbing VIII. MICROBIAL GENETICS 27. Regulation of Gene Expression 28. Isolation of Bacterial Mutants 29. Transformation of Bacteria 30. DNA Fingerprinting 31. Genetic Engineering 32. Ames Test for Detecting Possible Chemical Carcinogens IX. THE MICROBIAL WORLD 33. Fungi: Yeasts and Molds 34. Phototrophs: Algae and Cyanobacteria 35. Protozoa 36. Parasitic Helminths X. VIRUSES 37. Isolation and Titration of Bacteriophages 38. Plant Viruses XI. INTERACTION OF MICROBE AND HOST 39. Epidemiology 40. Koch’s Postulates XII. IMMUNOLOGY 41. Innate Immunology 42.  Agglutination Reactions: Slide Agglutination 43. Agglutination Reactions: Microtiter Agglutination 44. ELISA Technique XIII. MICROORGANISMS AND DISEASE 45. Bacteria of the Skin 46. Bacteria of the Respiratory Tract 47. Bacteria of the Mouth 48. Bacteria of the Gastrointestinal Tract 49. Bacteria of the Genitourinary Tract 50. Identification of an Unknown from a Clinical Sample 51. Rapid Identification Methods XIV. MICROBIOLOGY AND THE ENVIRONMENT 52. Microbes in Water: Multiple-Tube Technique 53. Microbes in Water: Membrane Filter Technique 54. Microbes in Food: Contamination 55. Microbes Used in the Production of Foods 56. Microbes in Soil: The Nitrogen and Sulfur Cycles 57. Microbes in Soil: Bioremediaton APPENDICES A. Pipetting B. Dilution Techniques and Calculations C. Use of the Spectrophotometer D. Graphing E. Use of the Dissecting Membrane F. Use of the Membrane Filter G. Electrophoresis H. Keys to Bacteria Index   

Comprehensive coverage of core microbiology topics including exercises in the areas of genetics and immunology.Laboratory Reports close out each exercise and help students learn to collect, record, and present data systematically.Consistently organized exercises include objectives; background definitions and explanations; lists of materials, supplies, and cultures needed; techniques required, with cross-references to previous exercises; step-by-step instructions; and space to record results.Questions embedded in the Background, Procedures, and Lab Report sections emphasize critical-thinking skills, requiring students to recall and apply information through analysis and logical reasoning.A Conclusions section consisting of a series of investigative questions is consistently included in the Lab Report of each exercise, encouraging students to closely analyze and understand their results from each laboratory exercise.100 photographs in full color, and numerous diagrams and drawings assist the student in visualizing techniques and interpreting results.An updated Preparation Guide written by the authors has specific instructions (i.e., recipes, materials) for preparing each lab, safety guidelines for handling blood and other bodily fluids, and page references to Tortora, Funke, and Case’s Microbiology: An Introduction, Eleventh edition. Tortora/Funke/Case, Microbiology: An Introduction and Johnson/Case, Laboratory Experimiments in Microbiology are available in the Pearson Custom Library, allowing instructors to create the perfect text with labs for their course. Select the chapters you need, in the sequence you want and delete chapters you don’t use: Your students pay only for the materials you choose. To create the perfect text, visit: http://www.pearsonlearningsolutions.com/custom-library/

The art program features precise illustrations in full-color. Full-color photographs appear alongside the relevant exercise, allowing students to clarify their experiments and better interpret their results. Twenty-five new photos have been included, and new illustrations have been added to Exercises 9, 11, 25, 26, 27, 29, 30, 40, and 44 to further illustrate and explain complex procedures.Expected Results or Hypothesis Section in each exercise’s Laboratory Report encourages students to speculate on the outcome of experimental procedures and to formulate a hypothesis when appropriate.Clinical Application questions, which have a clinical basis or are derived from a clinical situation, have been added to each Laboratory Report to help students draw connections between what they learn in lab and allied health careers.Prelab questions, multiple choice and true/false, are available online for laboratory instructors' use to ensure their students prepare adequately for each assigned laboratory exercise. Pre-lab questions are also available and assignable in MasteringMicrobiology®.Exercise on Transformation of Bacteria has been extensively revised to incorporate use of pGLO to introduce students to this important tool used in microbiology.Eye-catching full-color design engages the student while highlighting this manual’s trademark clear organization and presentation.