Molecular Biology Techniques: A Classroom Laboratory Manual, Fifth Edition, is an in-depth laboratory manual for upper-level undergraduates and beginning graduate students in the general field of molecular biology techniques and biochemical applications. Presented as a series of coordinated experiments that mimics either a one- or two-semester research experience, the book also provides theories and explanations behind the techniques. Topics include manipulation of recombinant DNA, protein expression and purification, analysis of mRNA levels, mammalian cell culture techniques, CRISPR-mediated gene knockdown, and more. New topics, such as Gibson assembly experiments, have also been included. For instructors, the book contains easy to follow guides and preparation lists, designed to facilitate lab setup and pre-lab questions for use as a teaching tool.
Les mer
1. DNA MANIPULATION Lab session 1-5
2. SCREENING TRANSFORMANTS Lab session 6-8
3. EXPRESSION, PURIFICATION, AND ANALYSIS OF RECOMBINANT PROTEINS FROM BACTERIA Lab session 9-12b
4. ANALYSIS OF mRNA LEVELS Lab Session 13-17
5. Modulation of Gene Expression Lab Session 18-30 PART VI In silico tools Lab Session 31-33
Les mer
An in-depth laboratory manual for undergraduates in the general field of molecular biology techniques and biochemical applications
Presents student-tested labs proven successful in real classroom laboratories
Covers updated lab sessions that focus on silico methods
Includes quizzes, discussion questions and further online resources for students, as well as lab preparation lists for instructor use
Also includes updates such as Gibson assembly experiments and lab session flow charts for easier selection
Les mer
Produktdetaljer
ISBN
9780443267147
Publisert
2026-03-30
Utgave
5. utgave
Utgiver
Elsevier Science Publishing Co Inc
Vekt
730 gr
Høyde
276 mm
Bredde
216 mm
Aldersnivå
U, 05
Språk
Product language
Engelsk
Format
Product format
Heftet
Antall sider
528