Sixteen chapters provide detailed methodological protocols on how to culture different types of neural tissue ... These chapters have been written by experts who have extensive experience with the methods in their own laboratories; hence, they typically reflect the product of years of experience with variants of the protocols described. The book is very accessible and easy to use. It is well organized, and indexed ... Nothing teaches like experience, and at least this book will give you some ideas about the experience of others that will help to guide your progress. For the neuroscientist engaged in neural cell culture this manual may be useful as a general reference, and for anyone who has immediate need for a particular chapter, this manual could indeed prove a very helpful resource.

R.E. Strecker, Harvard Medical School,

This practical book describes tried and tested methods for preparing cultures from a diverse range of tissues and cells from the central and peripheral nervous systems of mammals and birds.

Aslib Book Guide, vol.61, no.9, September 1996.

The complexity and cellular heterogeneity of the vertebrate nervous system makes it difficult to study neural processes at the cellular and molecular levels. The establishment of cell culture model systems for almost every region and cell type in the nervous system has therefore been crucial in facilitating such studies and in heralding some key breakthroughs in our understanding of the underlying mechanisms. Neural Cell Culture: A Practical Approach brings together methods for preparing cultures from a diverse range of neural tissues and cells, from both the central and peripheral nervous systems of mammalian and avain species. Important features of this volume are the clearly illustrated dissection guides accompanying each chapter, methods for enriching and purifying distinct cell types, and guidance on optimizing cell viability and growth. Each chapter includes valuable step-by-step protocols, hints, and tips for success, advice, and background information.
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This book brings together methods for preparing and maintaining cell cultures from a diverse range of neural tissues and cells, from both the central and peripheral nervous systems.
PART I - CNS NEURONS ; 1. Cerebellum: granule cells ; 2. Cerebellar Purkinje neurons ; 3. Nigral & straital neurons ; 4. Septal neurons ; 5. Proliferation & differentiation in retinal cell cultures ; 6. Purified embryonic motoneurones ; PART II - CNS GLIA AND NON-NEURONAL CELLS ; 7. Astrocytes ; 8. Oligodendrocyte lineage cells from neonatal rat brain ; 9. Micrglia: the tissue macrophage of the CNS ; 10. Brain endothelium ; PART III - PNS NEURONS AND GLIA ; 11. Neural crest cells: strategies to generate lineage diversifaction in vitro ; 13. Avian cilliary and lumbar sympathetic ganglion neurons ; 14. Enteric ganglion cells ; 15. Mouse olfactory epithelium ; 16. Schwann cell culture
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`Sixteen chapters provide detailed methodological protocols on how to culture different types of neural tissue ... These chapters have been written by experts who have extensive experience with the methods in their own laboratories; hence, they typically reflect the product of years of experience with variants of the protocols described. The book is very accessible and easy to use. It is well organized, and indexed ... Nothing teaches like experience, and at least this book will give you some ideas about the experience of others that will help to guide your progress. For the neuroscientist engaged in neural cell culture this manual may be useful as a general reference, and for anyone who has immediate need for a particular chapter, this manual could indeed prove a very helpful resource.' R.E. Strecker, Harvard Medical School, `This practical book describes tried and tested methods for preparing cultures from a diverse range of tissues and cells from the central and peripheral nervous systems of mammals and birds.' Aslib Book Guide, vol.61, no.9, September 1996.
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Wide variety of neuronal and glial cells covered Clearly illustrated dissection guide with each chapter Single volume covering a range of methods

Produktdetaljer

ISBN
9780199634842
Publisert
1996
Utgiver
Oxford University Press
Vekt
471 gr
Høyde
235 mm
Bredde
156 mm
Dybde
16 mm
Aldersnivå
P, 06
Språk
Product language
Engelsk
Format
Product format
Heftet
Antall sider
268

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