"...this book would certainly benefit readers who are new to the field as well as those more experienced as it serves as a convenient collection of tried and trusted methods...the book gives valuable background and insight to the various techniques associated with protein sequencing, which are often used by those providing services from within a core facility to the wider population of life science researchers." -Proteomics "A positive feature of this book is that the fact that minute details, which are essential for successful implementation of an analytical method are provided. This book can serve as a useful reference for laboratories which mainly utilize chemical methods for protein analysis." -Pharmaceutical Research From Reviews of the First Edition... "...full of valuable information..." -Biochemical Education "...provides very useful resources for those embarking on protein identification or characterization projects..." -FEBS Letters "...an extremely useful compendium of protein analysis protocols..." -Nahrung-Food "...well organized and accessible..." -Journal of the American Chemical Society "The overall result is a concise and highly informative laboratory manual aimed at both operators and users of protein analysis facilities providing an understanding of what techniques involve, how samples should be prepared and in what form and amounts they should be submitted to achieve the most successful data. This publication has certainly proved to be a valuable asset to my laboratory and I would recommend it not only to those in protein sequencing and mass spectrometry laboratories, but also anyone with protein chemistry applications and interests."-Molecular Biotechnology

Bryan John Smith updates his much-acclaimed first edition with new and updated techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to analysis of protein sequence information via the powerful new tools of bioinformatics. Comprehensive and up-to-date, Protein Sequencing Protocols, Second Edition, provides for both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination.
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Determination of the protein sequence is as important today as it was a half century ago, even though the techniques and purposes have changed over time. The first edition of Protein Sequencing Protocols was a “snapshot” of methods in use in protein biochemistry laboratories at the time, and this, the second edition, is likewise.
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Bryan John Smith updates his much-acclaimed first edition of Protein Sequencing Protocols with new and refreshed techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to the analysis of protein sequence information via the powerful new tools of bioinformatics. Each readily reproducible protocol contains detailed step-by-step instructions to ensure successful experimental results, along with notes on troubleshooting and how to avoid pitfalls. Valuable appendices summarize changes in mass arising from protein modification, as well as consensus sequences for protein modification.
Comprehensive and up-to-date, Protein Sequencing Protocols, Second Edition, provides both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination and thus help assure robust results.
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Produktdetaljer

ISBN
9781617372841
Publisert
2010-11-10
Utgave
2. utgave
Utgiver
Humana Press Inc.
Høyde
229 mm
Bredde
152 mm
Aldersnivå
Research, P, 06
Språk
Product language
Engelsk
Format
Product format
Heftet
Antall sider
12

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