This book covers a comprehensive range of topics, with a particular focus on the latest information regarding mesenchymal stem cells (MSCs). It is divided into 17 chapters, which explore various aspects of stem cell technology, such as the different types of stem cells and their isolation from different animal sources. The book also includes information about the culture and characteristics of MSCs, as well as their osteoinductive potential. In addition, the book provides protocols for the isolation of MSCs from bone marrow, fat tissue, and embryonic stem cells. It also discusses the application of bioengineered stem cell technologies in skin wound healing, burn wound healing, critical bone defect healing, large bone defect healing, corneal wound healing, and liver regeneration. The book is intended to serve as a valuable resource for undergraduate and postgraduate research scholars, teachers, biologists, and clinicians involved in animal treatment and research. It features over 150 high-quality color photographs illustrating various aspects of stem cell isolation, culture, morphology, characteristics, and application in surgical interventions.