About the Editor vii

Preface ix

Contributors xi

1. Reactive Nanocolloids for Nanotechnologies and Microsystems 1
Christian Pichot

2. Nanoparticles Comprising pH/Temperature-Responsive Amphiphilic Block Copolymers and Their Applications in Biotechnology 31
Peihong Ni

3. Evolution in Malaria Disease Detection: From Parasite Visualization to Colloidal-Based Rapid Diagnostic 65
Duangporn Polpanich, Pramuan Tangboriboonrat, and Abdelhamid Elaissari

4. Antigen-Antibody Interactions Detected by Quasi-Elastic Light Scattering and Electrophoretic Mobility Measurements – A New Concept for Latex Immunodiagnostic Test 95
Teresa Basinska and Stanislaw Slomkowski

5. Biospecific Reactions by Affinity Latexes from Diagnostics to Multiplex Assay 117
Haruma Kawaguchi

6. Fluorescent Colloidal Particles as Detection Tools in Biotechnology Systems 133
Igor Nabiev, Alyona Sukhanova, Mikhail Artemyev, and Vladimir Oleinikov

7. Biofunctionalized Magnetic Micro/Nanoparticles for Biosensing Technologies 169
Nicole Jaffrezic-Renault, Claude Martelet, Yann Chevolot, and Jean-Pierre Cloarec

8. Colloids, Biotechnology, and Microfluidics 199
Cecile Cottin-Bizonne and Rosaria Ferrigno

9. Gas Sensors Based on Ultrathin Films of Conducting Polymers and Nanocomposites 223
Manoj K. Ram, O¨zlem Yavuz, and Matt Aldissi

10. Force Measurements between Emulsion Droplets As a New Tool for Medical Diagnostics 247
Abdelhamid Elaissari, Céline Genty, and Olivier Mondain-Monval

11. Particles for Protein Analysis in Microfluidic Systems 275
Anne Le Nel, Myriam Taverna, and Jean-Louis Viovy

12. Semicarbazide/a-oxo Aldehyde Site-Specific Ligation Chemistry: From Peptide Microarrays to the Micropatterning of Polycarbonate or Titanium Oxide Using Silica Nanoparticles 299
Ouafâa El-Mahdi, Vianney Souplet, Olivier Carion, Clément Roux, Jean-Michel Garcia, Céline Maillet, Christophe Olivier, Oleg Melnyk and Jean-Olivier Durand

13. Electrical Detection of Antibodies from Human Serum Based on the Insertion of Gold-Labeled Secondary Antibodies into Microor Nanogaps 329
Lionel Marcon, Didier Stiévenard, and Oleg Melnyk

Index 353

Discover new and emerging applications of colloidal nanoparticles. Dr. Abdelhamid Elaissari, internationally respected author and researcher, reports on and analyzes a broad range of important findings from new studies on the use of colloidal nanoparticles in biomedical, food, and environmental diagnostics and analyses. Throughout the presentation, the book uses a blend of classical tools, including optical microscopy, atomic force microscopy, microsystems, and microfluidics, to help you take full advantage of colloidal nanoparticles for your own research and applications.

Among the key topics covered are:

• The evolution in malaria disease detection, from parasite visualization to colloidal-based rapid diagnostics

• Biospecific reactions by affinity latexes, from diagnostics to multiplex assays

• Fluorescent colloidal particles as detection tools in biotechnology systems

• Gas sensors based on ultrathin films of conducting polymers and nanocomposites

• Force measurements between emulsion droplets as a new tool for medical diagnostics

• Electrical detection of antibodies from human serum based on the insertion of gold-labeled secondary antibodies into microgaps and nanogaps

Special attention is given to the use of particles and reactive particles for in vitro biomedical diagnostics. In addition, the book explores many unusual nanoparticles with interesting and helpful applications. Biotechnological systems are presented and illustrated using examples of actual applications, helping you progress from theory to application.

The first book to present and analyze the great potential of colloidal nanoparticles across a broad range of biotechnology applications, this guide will give you the information you need to develop your own important applications and help you to harness these particles' full potential.