1. Purification of endogenous tDRs by hybridization-based pulldown Yasutoshi Akiyama 2. Northern blotting for human pre-tRNA and tRNA-derived RNAs Yasutoshi Akiyama 3. In vitro translation systems to study tDRs Pavel Ivanov 4. tRNA derived RNAs that form tetramolecular assemblies Prakash Kharel 5. Ribozyme-mediated expression of tRNA-derived small RNAs in bacteria Norbert Polacek 6. Ribosome-associated tDRs in yeast Norbert Polacek 7. tDR bioinformatics Todd Lowe 8. cP-RNA-seq for the specific sequencing of tRNA halves and other cP-containing RNAs Yohei Kirino 9. TaqMan RT-qPCR for specific quantification of tRNA halves and other small RNAs Yohei Kirino 10. Detection and isolation extracellular tDRs Alfonso Cayota 11. Preparation of nicked tRNAs Juan Pablo Tosar and Alfonso Cayota 12. In vitro functional analysis of plant tDRs Laurence Drouard 13. TGIRT-seq to profile tRNA-derived RNAs and associated RNA modifications Anindya Dutta, Zhangli Su, Abigail Johnston and Monima Anam 14. A general framework to over-express tRNA-derived RNAs from their parental tRNAs in mammalian cells Anindya Dutta, Zhangli Su, Kumarjeet Banerjee and Fatemeh Esmaeili 15. Functional characterization of tRNA-derived small RNAs in stem cells Cristian Bellodi 16. Detection of mitochondrial tDRs in killifish Claire Riggs, Gazal Kalyan and Jason Podrabsky 17. Measurement and comparison of full-length tRNA and tRF abundance and modifications Tao Pan and Luke Frietze 18. Using a Fluorescence based tRNA to image tDR biogenesis and localization. Saumya Das and Guoping Li 19. Manipulation of tDR expression using gain of function and loss of function approaches Saumya Das and Guoping Li 20. TBD Mark A. Kay and Hak Kyun Kim 21. Nicked tRNAs in bacteria Sandra Wolin 22. Determining Small RNA-Interacting Proteomes Using Endogenously Modified tRNA-Derived RNAs Matthias Ronald Schaefer, Vera Oberbauser and Aleksej Drino 23. Activation of angiogenin by ribosome to produce tRNA-derived RNAs Andrei A. Korostelev and Anna B. Loveland